Hotspot

trnK-UUU-rps16

LSC · 4498-5089 · 592 bp

Back to Salacia

Pi 0.018

MarkerSeek score 67.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 12
pi: 0.018
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GGGGCTCAACCTACAGAAAC	50.0	55.0	58.2	58.2	652-747 bp	1.0	82.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GGGGCTCAACCTACAGAAAC	50.0	55.0	58.2	58.2	651-746 bp	1.0	82.6
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	GGGGCTCAACCTACAGAAAC	50.0	55.0	57.5	58.2	638-733 bp	1.0	80.3
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	GGGGCTCAACCTACAGAAAC	50.0	55.0	58.6	58.2	663-758 bp	1.0	79.3
trnK-UUU-rps16_p5	TCGCACTTAAAAGCCGAGTA	GGGGCTCAACCTACAGAAAC	45.0	55.0	57.3	58.2	661-756 bp	1.0	78.1