Hotspot

trnH-GUG-psbA

LSC · 76-609 · 534 bp

Back to Salacia

Pi 0.0181

MarkerSeek score 73.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 14
pi: 0.0181
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	629-651 bp	1.0	83.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	643-665 bp	1.0	83.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	642-664 bp	1.0	83.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	644-666 bp	1.0	83.6
trnH-GUG-psbA_p5	GATCCACAATCCACTGCCTT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	649-671 bp	1.0	83.5