Hotspot

trnP-UGG

LSC · 67310-67383 · 74 bp

Back to Salacca

Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnP-UGG

Feature Metadata

label_name: trnP-UGG
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG_p1	ATCCCTCGTTACTGCCCATA	TGTGGAAAACAAGACAGGGA	50.0	45.0	57.9	56.9	162 bp	1.0	70.8
trnP-UGG_p2	TAATCCCTCGTTACTGCCCA	TGTGGAAAACAAGACAGGGA	50.0	45.0	58.1	56.9	164 bp	1.0	70.5
trnP-UGG_p3	TCGTTACTGCCCATAGGAGA	TGTGGAAAACAAGACAGGGA	50.0	45.0	57.6	56.9	157 bp	1.0	68.0
trnP-UGG_p4	ATCCCTCGTTACTGCCCATA	ATGTGGAAAACAAGACAGGGA	50.0	42.9	57.9	57.4	163 bp	1.0	67.0
trnP-UGG_p5	ATCCCTCGTTACTGCCCATA	TGTGGAAAACAAGACAGGGAT	50.0	42.9	57.9	57.4	162 bp	1.0	67.0