Hotspot

ndhF-rpl32

SSC · 114470-114863 · 394 bp

Back to Salacca

Pi 0.0305
MarkerSeek score 72.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
394
variable_sites
12
pi
0.0305
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT CCGATTTCGCTAAAGAAAAAGC 40.0 40.9 55.3 56.9 606-744 bp 1.0 41.4
ndhF-rpl32_p2 TTTGTTGCTGTCGGAATAAGT CCGATTTCGCTAAAGAAAAAGC 38.1 40.9 56.1 56.9 607-745 bp 1.0 41.4
ndhF-rpl32_p3 TTGTTGCTGTCGGAATAAGT CCGATTTCGCTAAAGAAAAAGCT 40.0 39.1 55.3 58.3 606-744 bp 1.0 41.4
ndhF-rpl32_p4 TTTGTTGCTGTCGGAATAAGT CCGATTTCGCTAAAGAAAAAGCT 38.1 39.1 56.1 58.3 607-745 bp 1.0 41.4
ndhF-rpl32_p5 TTGTTGCTGTCGGAATAAGT ACCGATTTCGCTAAAGAAAAAGC 40.0 39.1 55.3 58.5 607-745 bp 1.0 41.4