Hotspot

accD-psaI

LSC · 59463-60598 · 1136 bp

Back to Salacca

Pi 0.0205

MarkerSeek score 58.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 21
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGTACCCGATGGTTCAC	GGTCCCATCGGATCTAGACA	55.0	55.0	58.0	58.0	1411-1424 bp	1.0	85.2
accD-psaI_p2	GACAGTACCCGATGGTTCAC	TGGTCCCATCGGATCTAGAC	55.0	55.0	58.0	58.0	1412-1425 bp	1.0	85.2
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	1300-1313 bp	1.0	85.1
accD-psaI_p4	CCACGGTTTCTTTCCCTTGA	TGGTCCCATCGGATCTAGAC	50.0	55.0	58.0	58.0	1301-1314 bp	1.0	85.1
accD-psaI_p5	TCCACGGTTTCTTTCCCTTG	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	1301-1314 bp	1.0	85.1