Hotspot

rbcL-accD

LSC · 57169-57980 · 812 bp

Back to Salacca

Pi 0.00248

MarkerSeek score 60.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 2
pi: 0.00248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	1025-1030 bp	1.0	78.7
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	1027-1032 bp	1.0	78.7
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.2	58.1	1033-1038 bp	1.0	77.8
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1036-1041 bp	1.0	77.6
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1037-1042 bp	1.0	77.6