Hotspot

psaA-ycf3

LSC · 41876-42511 · 636 bp

Back to Salacca

Pi 0.00314

MarkerSeek score 60.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 2
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTGGCCCATTCTTCGAAAGA	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	752 bp	1.0	78.0
psaA-ycf3_p2	TCTGGCCCATTCTTCGAAAG	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	753 bp	1.0	78.0
psaA-ycf3_p3	CTGGCCCATTCTTCGAAAGA	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	756 bp	1.0	77.8
psaA-ycf3_p4	TCTGGCCCATTCTTCGAAAG	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	757 bp	1.0	77.8
psaA-ycf3_p5	TGGCCCATTCTTCGAAAGAC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.2	57.8	751 bp	1.0	77.5