Hotspot

rps14-psaB

LSC · 37265-37392 · 128 bp

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Pi 0.0367

MarkerSeek score 71.50

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 4
pi: 0.0367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	GTTCCAATTTCTGCCGCTTC	TTGATTGCCTCGACATCAGG	50.0	50.0	58.0	58.0	201-213 bp	1.0	85.2
rps14-psaB_p2	TCCAATTTCTGCCGCTTCTT	TTGATTGCCTCGACATCAGG	45.0	50.0	58.1	58.0	199-211 bp	1.0	84.6
rps14-psaB_p3	TTCCAATTTCTGCCGCTTCT	TTGATTGCCTCGACATCAGG	45.0	50.0	58.1	58.0	200-212 bp	1.0	84.6
rps14-psaB_p4	GTTCCAATTTCTGCCGCTTC	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.0	58.1	285-297 bp	1.0	84.3
rps14-psaB_p5	TCCAATTTCTGCCGCTTCTT	CCAGTGGCTCTTTCCATTGT	45.0	50.0	58.1	58.1	283-295 bp	1.0	83.7