Hotspot

psbM-trnD-GUC

LSC · 29766-30815 · 1050 bp

Back to Salacca

Pi 0.0576
MarkerSeek score 75.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1050
variable_sites
60
pi
0.0576
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GTCTCACTTGGGCTGCTTTA AGTTCAATCGGTCAGAGCAC 50.0 50.0 57.8 57.9 1789-1796 bp 1.0 81.1
psbM-trnD-GUC_p2 GTCTCACTTGGGCTGCTTTA AATTCTTCCTGGGTCGATGC 50.0 50.0 57.8 58.0 2271-2291 bp 1.0 80.9
psbM-trnD-GUC_p3 GTCTCACTTGGGCTGCTTTA TGGGGGTAGAGAGGAAGTTG 50.0 55.0 57.8 58.0 2319-2339 bp 1.0 80.8
psbM-trnD-GUC_p4 ACTTGGGCTGCTTTAATGGT AATTCTTCCTGGGTCGATGC 45.0 50.0 57.7 58.0 2266-2286 bp 1.0 79.9
psbM-trnD-GUC_p5 GTCTCACTTGGGCTGCTTTA TCTCTTTCAAGGAGGCAACG 50.0 50.0 57.8 57.8 2355-2375 bp 1.0 79.8