Hotspot

trnC-GCA-petN

LSC · 27971-29079 · 1109 bp

Back to Salacca

Pi 0.00451

MarkerSeek score 61.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1109
variable_sites: 5
pi: 0.00451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTGTATCAGATTTGGCGGCA	TAAAGCAGCCCAAGTGAGAC	45.0	50.0	57.9	57.8	1225-1226 bp	1.0	77.9
trnC-GCA-petN_p2	TTGTATCAGATTTGGCGGCA	ACCATTAAAGCAGCCCAAGT	45.0	45.0	57.9	57.7	1230-1231 bp	1.0	76.9
trnC-GCA-petN_p3	TGTATCAGATTTGGCGGCAT	TAAAGCAGCCCAAGTGAGAC	45.0	50.0	57.7	57.8	1224-1225 bp	1.0	76.1
trnC-GCA-petN_p4	TATCAGATTTGGCGGCATGG	TAAAGCAGCCCAAGTGAGAC	50.0	50.0	58.4	57.8	1222-1223 bp	1.0	75.8
trnC-GCA-petN_p5	TGTATCAGATTTGGCGGCAT	ACCATTAAAGCAGCCCAAGT	45.0	45.0	57.7	57.7	1229-1230 bp	1.0	75.1