Hotspot

ycf2

IRa · 168826-175890 · 7065 bp

Back to Sagittaria

Pi 0.000142

MarkerSeek score 53.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 7065
variable_sites: 1
pi: 0.000142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGCATCGAACACTGGTTGAA	GATCCACTGCGGGAATGATT	45.0	50.0	58.0	58.0	796-846 bp	1.0	83.6
ycf2_p2	TCATGGATCTCTCGGTTCGA	GATCCACTGCGGGAATGATT	50.0	50.0	57.9	58.0	614-664 bp	1.0	83.5
ycf2_p3	TCATGGATCTCTCGGTTCGA	CAGGCAATCGGAAAAGCAAG	50.0	50.0	57.9	58.0	1076-1126 bp	1.0	82.7
ycf2_p4	TGCATCGAACACTGGTTGAA	CAGGCAATCGGAAAAGCAAG	45.0	50.0	58.0	58.0	1258-1308 bp	1.0	82.4
ycf2_p5	CGGCCCATTCGTATTCGTAT	CAGGCAATCGGAAAAGCAAG	50.0	50.0	57.9	58.0	1124-1174 bp	1.0	82.4