Hotspot

rpoC2

LSC · 20909-25642 · 4734 bp

Back to Sagittaria

Pi 0.00343

MarkerSeek score 56.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4734
variable_sites: 16
pi: 0.00343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTATATGGGGCTAGGGGTCG	TCCCGAGGAAGTGCATATCT	55.0	50.0	58.1	57.9	852-900 bp	1.0	84.3
rpoC2_p2	ATGTCGGGTAAGTGAGGGAA	TCCCGAGGAAGTGCATATCT	50.0	50.0	58.1	57.9	1261-1309 bp	1.0	83.9
rpoC2_p3	TGTCGGGTAAGTGAGGGAAT	TCCCGAGGAAGTGCATATCT	50.0	50.0	58.1	57.9	1260-1308 bp	1.0	83.9
rpoC2_p4	TTATATGGGGCTAGGGGTCG	TTTCATTCCCGAGGAAGTGC	55.0	50.0	58.1	58.2	858-906 bp	1.0	83.6
rpoC2_p5	TGTCGGGTAAGTGAGGGAAT	TTTCATTCCCGAGGAAGTGC	50.0	50.0	58.1	58.2	1266-1314 bp	1.0	83.1