Hotspot

ccsA

SSC · 136206-137174 · 969 bp

Back to Sagittaria

Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AATAGAAAAGGAGCGCAAGA	ACTGAGCGATGAAATGGAGT	40.0	45.0	55.1	56.9	1169 bp	1.0	48.0
ccsA_p2	AATAGAAAAGGAGCGCAAGA	CGTGAGAGTGATGTAGAGGG	40.0	55.0	55.1	56.9	1111 bp	1.0	47.4
ccsA_p3	AAATAGAAAAGGAGCGCAAGA	ACTGAGCGATGAAATGGAGT	38.1	45.0	55.9	56.9	1170 bp	1.0	46.5
ccsA_p4	AGAAAAGGAGCGCAAGATATTC	ACTGAGCGATGAAATGGAGT	40.9	45.0	56.8	56.9	1166 bp	1.0	46.3
ccsA_p5	GAAAAGGAGCGCAAGATATTCT	ACTGAGCGATGAAATGGAGT	40.9	45.0	56.8	56.9	1165 bp	1.0	46.3