Hotspot

ycf1-trnN-GUU

IRa · 152631-153434 · 804 bp

Back to Sagittaria

Pi 0
MarkerSeek score 63.00
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
804
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 CAGAATGAGCTCAGGAACCC CTACGGCCAATCGGTTAACA 55.0 50.0 58.0 58.0 904-962 bp 1.0 83.6
ycf1-trnN-GUU_p2 CAGAATGAGCTCAGGAACCC TACGGCCAATCGGTTAACAG 55.0 50.0 58.0 58.0 903-961 bp 1.0 83.6
ycf1-trnN-GUU_p3 CAGAATGAGCTCAGGAACCC ATTCGAACCTACGGCCAATC 55.0 50.0 58.0 58.1 912-970 bp 1.0 83.3
ycf1-trnN-GUU_p4 CAGAATGAGCTCAGGAACCC GATTCGAACCTACGGCCAAT 55.0 50.0 58.0 58.1 913-971 bp 1.0 83.3
ycf1-trnN-GUU_p5 CAGAATGAGCTCAGGAACCC GTAGGATTCGAACCTACGGC 55.0 55.0 58.0 57.9 917-975 bp 1.0 82.7