Hotspot

ndhF-rpl32

SSC · 133902-135251 · 1350 bp

Back to Sagittaria

Pi 0
MarkerSeek score 58.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1350
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAACAAGCAGGAGTCCAAA ACGAAAAAGCTTTAACGGCG 50.0 45.0 58.0 58.0 1538-1539 bp 1.0 79.7
ndhF-rpl32_p2 AGCAGGAGTCCAAACCCTAT ACGAAAAAGCTTTAACGGCG 50.0 45.0 58.0 58.0 1532-1533 bp 1.0 79.6
ndhF-rpl32_p3 TCCAAACCCTATTGCCATCG ACGAAAAAGCTTTAACGGCG 50.0 45.0 57.9 58.0 1524-1525 bp 1.0 79.4
ndhF-rpl32_p4 GGAGTCCAAACCCTATTGCC ACGAAAAAGCTTTAACGGCG 55.0 45.0 58.2 58.0 1528-1529 bp 1.0 77.9
ndhF-rpl32_p5 CCAAACCCTATTGCCATCGT ACGAAAAAGCTTTAACGGCG 50.0 45.0 58.2 58.0 1523-1524 bp 1.0 77.9