Hotspot

trnN-GUU-ycf1

IRb · 122258-123061 · 804 bp

Back to Sagittaria

Pi 0
MarkerSeek score 63.00
Primer available yes
Label trnN-GUU-ycf1

Feature Metadata

label_name
trnN-GUU-ycf1
feature_type
igs
region
IRb
length_bp
804
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ycf1_p1 CTACGGCCAATCGGTTAACA CAGAATGAGCTCAGGAACCC 50.0 55.0 58.0 58.0 904-962 bp 1.0 83.6
trnN-GUU-ycf1_p2 TACGGCCAATCGGTTAACAG CAGAATGAGCTCAGGAACCC 50.0 55.0 58.0 58.0 903-961 bp 1.0 83.6
trnN-GUU-ycf1_p3 ATTCGAACCTACGGCCAATC CAGAATGAGCTCAGGAACCC 50.0 55.0 58.1 58.0 912-970 bp 1.0 83.3
trnN-GUU-ycf1_p4 GATTCGAACCTACGGCCAAT CAGAATGAGCTCAGGAACCC 50.0 55.0 58.1 58.0 913-971 bp 1.0 83.3
trnN-GUU-ycf1_p5 GTAGGATTCGAACCTACGGC CAGAATGAGCTCAGGAACCC 55.0 55.0 57.9 58.0 917-975 bp 1.0 82.7