Hotspot

trnF-GAA-ndhJ

LSC · 57597-58251 · 655 bp

Back to Sagittaria

Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GTTGGATCGGTTGGCCTTTA	50.0	50.0	58.1	58.2	760 bp	1.0	78.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGGATCGGTTGGCCTTTAC	50.0	50.0	58.1	58.2	759 bp	1.0	78.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GATCGGTTGGCCTTTACGTA	50.0	50.0	58.1	57.7	756 bp	1.0	77.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTCATCCGCGACTTAAACG	50.0	50.0	58.1	58.4	797 bp	1.0	76.7
trnF-GAA-ndhJ_p5	TCGGGATAGCTCAGTTGGTA	TTGGATCGGTTGGCCTTTAC	50.0	50.0	57.6	58.2	797 bp	1.0	75.2