Pi
0
MarkerSeek score
56.50
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AAAGCCAGCTATCGGAATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 2686-2688 bp | 1.0 | 79.0 |
| trnT-UGU-trnL-UAA_p2 | AAAGCCAGCTATCGGAATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 2694-2696 bp | 1.0 | 78.5 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 2673-2675 bp | 1.0 | 78.1 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 2681-2683 bp | 1.0 | 77.6 |
| trnT-UGU-trnL-UAA_p5 | AAATGCGATGCTCTAACCCC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.3 | 58.0 | 2645-2647 bp | 1.0 | 77.5 |