Hotspot

psbM-trnD-GUC

LSC · 35878-36740 · 863 bp

Back to Sagittaria

Pi 0.00116

MarkerSeek score 59.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 1
pi: 0.00116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTGACTTCCA	TATTGTACGGCTCATGGGGA	40.0	50.0	54.5	58.2	974 bp	1.0	50.3
psbM-trnD-GUC_p2	TGCGAGAATATTGACTTCCA	GCTCATGGGGACTGTAGTTC	40.0	55.0	54.5	57.7	965 bp	1.0	49.4
psbM-trnD-GUC_p3	TGCGAGAATATTGACTTCCA	AGTTCAATTGGTCAGAGCGT	40.0	45.0	54.5	57.4	950 bp	1.0	47.5
psbM-trnD-GUC_p4	TGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCGTCG	40.0	50.0	54.5	58.6	948 bp	1.0	47.4
psbM-trnD-GUC_p5	TGCGAGAATATTGACTTCCAT	TATTGTACGGCTCATGGGGA	38.1	50.0	55.1	58.2	974 bp	1.0	47.2