Hotspot

rpl33-rps18

LSC · 67080-67341 · 262 bp

Back to Sagina

Pi 0.0262
MarkerSeek score 62.00
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
262
variable_sites
13
pi
0.0262
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CAAACATACAATTCATGGAGAGA GCAAACGCCTACGAACAGAT 34.8 50.0 54.7 58.9 345-359 bp 1.0 45.0
rpl33-rps18_p2 CAAACATACAATTCATGGAGAGA CAAACGCCTACGAACAGATC 34.8 50.0 54.7 56.9 344-358 bp 1.0 45.0
rpl33-rps18_p3 CAAACATACAATTCATGGAGAGA TAATCAATTCGATCCCCCGG 34.8 50.0 54.7 57.1 376-390 bp 1.0 44.6
rpl33-rps18_p4 CAAACATACAATTCATGGAGAGA AATCAATTCGATCCCCCGGT 34.8 50.0 54.7 59.2 375-389 bp 1.0 44.6
rpl33-rps18_p5 CAAACATACAATTCATGGAGAGA ATCAATTCGATCCCCCGGTT 34.8 50.0 54.7 59.2 374-388 bp 1.0 44.6