Hotspot

psbL-psbF

LSC · 63506-63532 · 27 bp

Back to Sagina

Pi 0.0432
MarkerSeek score 71.80
Primer available yes
Label psbL-psbF

Feature Metadata

label_name
psbL-psbF
feature_type
igs
region
LSC
length_bp
27
variable_sites
2
pi
0.0432
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-psbF_p1 TTCGGGTTTGATTGTGTCGT TGGGGTCAATATCCGCAATG 45.0 50.0 58.0 58.0 85-86 bp 1.0 81.4
psbL-psbF_p2 TCGTTCGGGTTTGATTGTGT TGGGGTCAATATCCGCAATG 45.0 50.0 58.0 58.0 88-89 bp 1.0 81.3
psbL-psbF_p3 CGTTCGGGTTTGATTGTGTC TGGGGTCAATATCCGCAATG 50.0 50.0 57.7 58.0 87-88 bp 1.0 79.2
psbL-psbF_p4 GTTCGGGTTTGATTGTGTCG TGGGGTCAATATCCGCAATG 50.0 50.0 57.7 58.0 86-87 bp 1.0 79.2
psbL-psbF_p5 TTCGGGTTTGATTGTGTCGT TTTTGGGGTCAATATCCGCA 45.0 45.0 58.0 57.5 88-89 bp 1.0 77.3