Hotspot

petA-psbJ

LSC · 62153-63117 · 965 bp

Back to Sagina

Pi 0.0185
MarkerSeek score 63.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
965
variable_sites
31
pi
0.0185
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGCGTACAAGGTCTTTTGT TTCTGGATTGGGATCATCGC 45.0 50.0 58.1 57.7 1082-1102 bp 1.0 81.3
petA-psbJ_p2 ACGCGTACAAGGTCTTTTGT TCTGGATTGGGATCATCGCT 45.0 50.0 58.1 58.6 1081-1101 bp 1.0 78.8
petA-psbJ_p3 ACGCGTACAAGGTCTTTTGT CTGGATTGGGATCATCGCTA 45.0 50.0 58.1 56.9 1080-1100 bp 1.0 74.3
petA-psbJ_p4 ACGCGTACAAGGTCTTTTGT ATTCTGGATTGGGATCATCGC 45.0 47.6 58.1 58.2 1083-1103 bp 1.0 73.7
petA-psbJ_p5 CGCGTACAAGGTCTTTTGTT TTCTGGATTGGGATCATCGC 45.0 50.0 57.0 57.7 1081-1101 bp 1.0 73.5