Hotspot

ndhC-trnV-UAC

LSC · 49868-50808 · 941 bp

Back to Sagina

Pi 0.0172
MarkerSeek score 62.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
941
variable_sites
31
pi
0.0172
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATGTCATATTCA CCGAGAAAGTCTACGGTTCG 36.4 55.0 56.0 58.1 1013-1139 bp 1.0 49.9
ndhC-trnV-UAC_p2 AAAATGCCCAGAAAATGTCA CCGAGAAAGTCTACGGTTCG 35.0 55.0 53.9 58.1 1017-1143 bp 1.0 48.9
ndhC-trnV-UAC_p3 AAAAATGCCCAGAAAATGTCA CCGAGAAAGTCTACGGTTCG 33.3 55.0 54.8 58.1 1018-1144 bp 1.0 47.9
ndhC-trnV-UAC_p4 TGCCCAGAAAATGTCATATTCA ACTGCATGTTGGGTCTTTGA 36.4 45.0 56.0 57.6 1078-1204 bp 1.0 47.7
ndhC-trnV-UAC_p5 AAATGCCCAGAAAATGTCAT CCGAGAAAGTCTACGGTTCG 35.0 55.0 53.7 58.1 1016-1142 bp 1.0 46.9