Hotspot

psaA-ycf3

LSC · 41040-42179 · 1140 bp

Pi 0.0164

MarkerSeek score 65.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1140
variable_sites: 32
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCGGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1246-1342 bp	1.0	85.0
psaA-ycf3_p2	GTTCTTGAAAAATGGCCGGG	TTGGTTGAAGATCACGGGAC	50.0	50.0	57.9	57.8	1179-1275 bp	1.0	84.0
psaA-ycf3_p3	TTCTTGAAAAATGGCCGGGT	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.3	58.0	1245-1341 bp	1.0	83.3
psaA-ycf3_p4	GTTCTTGAAAAATGGCCGGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	1242-1338 bp	1.0	83.3
psaA-ycf3_p5	GTTCTTGAAAAATGGCCGGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	1244-1340 bp	1.0	83.2