Hotspot

rpoB-trnC-GCA

LSC · 26310-27239 · 930 bp

Back to Sagina

Pi 0.0201
MarkerSeek score 49.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
930
variable_sites
34
pi
0.0201
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGAGCCCATATCCTTGCTTT AGTATATTACCCCGCACCCA 50.0 50.0 58.0 57.9 1769-1787 bp 1.0 81.6
rpoB-trnC-GCA_p2 CCCCTACACAAGCAAACTGT AGTATATTACCCCGCACCCA 50.0 50.0 58.0 57.9 1960-1978 bp 1.0 81.5
rpoB-trnC-GCA_p3 CCCCTACACAAGCAAACTGT TATTACCCCGCACCCAATTG 50.0 50.0 58.0 57.9 1963 bp 0.25 49.4
rpoB-trnC-GCA_p4 CGAGCCCATATCCTTGCTTT TATTACCCCGCACCCAATTG 50.0 50.0 58.0 57.9 1772 bp 0.25 49.3
rpoB-trnC-GCA_p5 CCGGGGCTTTGCAATATTTG TATTACCCCGCACCCAATTG 50.0 50.0 58.1 57.9 1652 bp 0.25 49.0