Hotspot

rps16-trnQ-UUG

LSC · 6040-6715 · 676 bp

Back to Sagina

Pi 0.0331
MarkerSeek score 66.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
676
variable_sites
40
pi
0.0331
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 728-779 bp 1.0 85.8
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.2 58.2 739-790 bp 1.0 85.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GTAAGGCATCGGGTTTTGGT 45.0 50.0 58.1 58.5 729-780 bp 1.0 83.5
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTGGTAAGGCATCGGGTTTT 45.0 50.0 58.1 58.5 732-783 bp 1.0 83.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TGGTAAGGCATCGGGTTTTG 45.0 50.0 58.1 58.5 731-782 bp 1.0 83.5