Hotspot

ndhH

SSC · 117199-118380 · 1182 bp

Back to Saccharum

Pi 0.00132

MarkerSeek score 50.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 7
pi: 0.00132
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TAGGAGGGCCAAAATGGGTA	TGCATTTTGATGAGCCTCGT	50.0	45.0	58.0	58.2	1367 bp	1.0	83.0
ndhH_p2	CAGTAGGAGGGCCAAAATGG	TGCATTTTGATGAGCCTCGT	55.0	45.0	58.2	58.2	1370 bp	1.0	81.2
ndhH_p3	CCAGTAGGAGGGCCAAAATG	TGCATTTTGATGAGCCTCGT	55.0	45.0	58.2	58.2	1371 bp	1.0	81.2
ndhH_p4	GAGGGCCAAAATGGGTAGAA	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.5	58.2	1364 bp	1.0	78.9
ndhH_p5	TAGGAGGGCCAAAATGGGTA	TTGCATTTTGATGAGCCTCG	50.0	45.0	58.0	57.1	1368 bp	1.0	77.0