Hotspot

ndhF-rpl32

SSC · 108028-108882 · 855 bp

Back to Saccharum

Pi 0.00353

MarkerSeek score 50.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 855
variable_sites: 10
pi: 0.00353
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	962-968 bp	1.0	46.5
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	934-940 bp	1.0	46.3
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	935-941 bp	1.0	46.3
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	936-942 bp	1.0	46.3
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	937-943 bp	1.0	46.3