Hotspot

rps11-rpl36

LSC · 78339-78523 · 185 bp

Back to Saccharum

Pi 0.0027

MarkerSeek score 49.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 3
pi: 0.0027
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGCAAACCAATACGCACTTT	CGTGGGCGAATTAGAGTCAT	45.0	50.0	58.0	57.8	287-303 bp	1.0	81.6
rps11-rpl36_p2	CTACGCAAACCAATACGCAC	CGTGGGCGAATTAGAGTCAT	50.0	50.0	57.8	57.8	290-306 bp	1.0	80.7
rps11-rpl36_p3	TACGCAAACCAATACGCACT	CGTGGGCGAATTAGAGTCAT	45.0	50.0	58.2	57.8	289-305 bp	1.0	80.3
rps11-rpl36_p4	TCTACGTGCACTCTTCCGTA	CGTGGGCGAATTAGAGTCAT	50.0	50.0	58.2	57.8	327-343 bp	1.0	80.0
rps11-rpl36_p5	CGTGCACTCTTCCGTAAACT	CGTGGGCGAATTAGAGTCAT	50.0	50.0	58.2	57.8	323-339 bp	1.0	79.8