Hotspot

rpl33-rps18

LSC · 67855-68166 · 312 bp

Back to Saccharum

Pi 0.00214

MarkerSeek score 46.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 3
pi: 0.00214
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACGCCCGGACAATTAGAATT	TGGATTTACGAAAGGGTTGCT	45.0	42.9	57.6	57.9	428-445 bp	1.0	71.3
rpl33-rps18_p2	ACAATACGCCCGGACAATTA	TGGATTTACGAAAGGGTTGCT	45.0	42.9	57.3	57.9	433-450 bp	1.0	69.0
rpl33-rps18_p3	AAAGAATCGCCACAATACGC	TGGATTTACGAAAGGGTTGCT	45.0	42.9	57.2	57.9	444-461 bp	1.0	67.9
rpl33-rps18_p4	CACAATACGCCCGGACAATT	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.9	57.9	434-451 bp	1.0	67.3
rpl33-rps18_p5	ACGCCCGGACAATTAGAATT	TGGATTTACGAAAGGGTTGC	45.0	45.0	57.6	56.3	428-445 bp	1.0	67.1