Hotspot

trnC-GCA-rpoB

LSC · 20707-21918 · 1212 bp

Back to Saccharum

Pi 0.001

MarkerSeek score 47.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 5
pi: 0.001
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1353-1381 bp	1.0	79.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1351-1379 bp	1.0	79.2
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1354-1382 bp	1.0	79.2
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCGCCAAGCCTTGATTAA	50.0	45.0	58.1	57.8	1341-1369 bp	1.0	78.9
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CTTCCGCCAAGCCTTGATTA	50.0	50.0	58.1	58.2	1342-1370 bp	1.0	78.6