Hotspot

ilvH

LSC · 47470-48072 · 603 bp

Back to Saccharina

Pi 0.0293

MarkerSeek score 54.70

Primer available yes

Label ilvH

Feature Metadata

label_name: ilvH
feature_type: gene
region: LSC
length_bp: 603
variable_sites: 31
pi: 0.0293
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ilvH_p1	GAGGTAATGTCGCTCGGAAC	TGCAAATCGTGAGGGTTCAA	55.0	45.0	58.4	58.0	1593-1607 bp	1.0	80.7
ilvH_p2	GAGGTAATGTCGCTCGGAAC	CCGTTGATGCAAATCGTGAG	55.0	50.0	58.4	57.8	1600-1614 bp	1.0	79.6
ilvH_p3	GAGGTAATGTCGCTCGGAAC	CGTTGATGCAAATCGTGAGG	55.0	50.0	58.4	57.8	1599-1613 bp	1.0	79.6
ilvH_p4	GAGGTAATGTCGCTCGGAAC	TCCGTTGATGCAAATCGTGA	55.0	45.0	58.4	58.2	1601-1615 bp	1.0	79.3
ilvH_p5	CGCTCGGAACCGTAGAAATA	TGCAAATCGTGAGGGTTCAA	50.0	45.0	57.5	58.0	1583 bp	0.5	56.0