Hotspot

ftsH-psbH

SSC · 105464-105555 · 92 bp

Back to Saccharina

Pi 0.0743
MarkerSeek score 73.20
Primer available yes
Label ftsH-psbH

Feature Metadata

label_name
ftsH-psbH
feature_type
igs
region
SSC
length_bp
92
variable_sites
12
pi
0.0743
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ftsH-psbH_p1 ACTGGGTTACCTAAGAAAACT ACTTATGGGTATCGCTATGCT 38.1 42.9 54.1 56.4 247-252 bp 1.0 45.5
ftsH-psbH_p2 ACTGGGTTACCTAAGAAAACT ACTTATGGGTATCGCTATGC 38.1 45.0 54.1 54.8 247-252 bp 1.0 45.5
ftsH-psbH_p3 ACTGGGTTACCTAAGAAAAC ACTTATGGGTATCGCTATGCT 40.0 42.9 52.4 56.4 247-252 bp 1.0 45.5
ftsH-psbH_p4 ACTGGGTTACCTAAGAAAACT CTTATGGGTATCGCTATGCT 38.1 45.0 54.1 54.5 246-251 bp 1.0 45.5
ftsH-psbH_p5 ACTGGGTTACCTAAGAAAACTA ACTTATGGGTATCGCTATGCT 36.4 42.9 54.1 56.4 247-252 bp 1.0 45.5