Hotspot

trnL2-rns

IRa · 81991-82526 · 536 bp

Back to Saccharina

Pi 0.037

MarkerSeek score 68.10

Primer available yes

Label trnL2-rns

Feature Metadata

label_name: trnL2-rns
feature_type: igs
region: IRa
length_bp: 536
variable_sites: 33
pi: 0.037
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL2-rns_p1	GTTCAAGTCTCTCCGTTCGT	GCATGTGTAAAGCATACCGC	50.0	50.0	57.6	57.9	536-607 bp	1.0	84.3
trnL2-rns_p2	GTTCAAGTCTCTCCGTTCGT	TTCGTACGACTTGCATGTGT	50.0	45.0	57.6	57.9	548-619 bp	1.0	83.9
trnL2-rns_p3	CAGACGCGCTAGATTTAGGT	GCATGTGTAAAGCATACCGC	50.0	50.0	57.5	57.9	578-649 bp	1.0	83.4
trnL2-rns_p4	GTTCAAGTCTCTCCGTTCGT	ACACTTTCGTACGACTTGCA	50.0	45.0	57.6	57.8	553-624 bp	1.0	83.3
trnL2-rns_p5	CAGACGCGCTAGATTTAGGT	TTCGTACGACTTGCATGTGT	50.0	45.0	57.5	57.9	590-661 bp	1.0	83.0