Hotspot

psbY-rpl32

LSC · 81258-81597 · 340 bp

Back to Saccharina

Pi 0.0328

MarkerSeek score 64.30

Primer available yes

Label psbY-rpl32

Feature Metadata

label_name: psbY-rpl32
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 20
pi: 0.0328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbY-rpl32_p1	TGTGTTAGCAGCAGCATCTT	CTTCCCTTTGCTTTTGAGCG	45.0	50.0	57.8	57.9	457-463 bp	1.0	82.9
psbY-rpl32_p2	CTGTGTTAGCAGCAGCATCT	CTTCCCTTTGCTTTTGAGCG	50.0	50.0	58.3	57.9	458-464 bp	1.0	82.3
psbY-rpl32_p3	TGTGTTAGCAGCAGCATCTT	CTTTGCTTTTGAGCGCCTTT	45.0	45.0	57.8	58.1	452-458 bp	1.0	82.3
psbY-rpl32_p4	CTGTGTTAGCAGCAGCATCT	CTTTGCTTTTGAGCGCCTTT	50.0	45.0	58.3	58.1	453-459 bp	1.0	81.6
psbY-rpl32_p5	TGTGTTAGCAGCAGCATCTT	TATCAGCTGCTGTACGTCCT	45.0	50.0	57.8	58.2	504-510 bp	1.0	81.0