Hotspot

psbA-cbbx

LSC · 74530-75283 · 754 bp

Back to Saccharina

Pi 0.0544

MarkerSeek score 76.30

Primer available yes

Label psbA-cbbx

Feature Metadata

label_name: psbA-cbbx
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 72
pi: 0.0544
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-cbbx_p1	CTGCTCCTTCTGTTGTTGGT	ACAGCTAGTAGTGTATTTAGTT	50.0	31.8	58.0	52.0	760-804 bp	1.0	51.3
psbA-cbbx_p2	CTGCTCCTTCTGTTGTTGGT	ACAGCTAGTAGTGTATTTAGTTT	50.0	30.4	58.0	52.9	760-804 bp	1.0	51.3
psbA-cbbx_p3	TGTTGCGATTTCTGCTCCTT	ACAGCTAGTAGTGTATTTAGTT	45.0	31.8	58.1	52.0	771-815 bp	1.0	51.2
psbA-cbbx_p4	TTCCTGTTGCGATTTCTGCT	ACAGCTAGTAGTGTATTTAGTT	45.0	31.8	58.1	52.0	775-819 bp	1.0	51.2
psbA-cbbx_p5	TGTTGCGATTTCTGCTCCTT	ACAGCTAGTAGTGTATTTAGTTT	45.0	30.4	58.1	52.9	771-815 bp	1.0	51.2