Hotspot

psaC-psbA

LSC · 73007-73443 · 437 bp

Back to Saccharina

Pi 0.0686

MarkerSeek score 66.80

Primer available yes

Label psaC-psbA

Feature Metadata

label_name: psaC-psbA
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 51
pi: 0.0686
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-psbA_p1	AGGACAAGCACGAACACATT	AGGAGGTGCTGCGATAAAAG	45.0	50.0	58.0	58.0	677 bp	1.0	85.8
psaC-psbA_p2	GGACAAGCACGAACACATTG	AGGAGGTGCTGCGATAAAAG	50.0	50.0	58.0	58.0	676 bp	1.0	85.7
psaC-psbA_p3	TAATTGGGTCACCTGGGACT	AGGAGGTGCTGCGATAAAAG	50.0	50.0	57.9	58.0	1003 bp	1.0	83.9
psaC-psbA_p4	AGGACAAGCACGAACACATT	ATGAACCACCGAAAACACCA	45.0	45.0	58.0	57.9	1134 bp	1.0	83.1
psaC-psbA_p5	GGACAAGCACGAACACATTG	ATGAACCACCGAAAACACCA	50.0	45.0	58.0	57.9	1133 bp	1.0	83.0