Hotspot

trnL1-trnC

LSC · 48926-49013 · 88 bp

Back to Saccharina

Pi 0.0568

MarkerSeek score 68.30

Primer available yes

Label trnL1-trnC

Feature Metadata

label_name: trnL1-trnC
feature_type: igs
region: LSC
length_bp: 88
variable_sites: 8
pi: 0.0568
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL1-trnC_p1	TTAAGTCCGTTGCGTCTACC	TAAGGCCGTGGATTGCAAAT	50.0	45.0	57.6	58.2	174-175 bp	1.0	80.1
trnL1-trnC_p2	TTAAGTCCGTTGCGTCTACC	CCAGTTCGAATCTGGGTGTC	50.0	55.0	57.6	58.3	145-146 bp	1.0	79.9
trnL1-trnC_p3	TTAAGTCCGTTGCGTCTACC	CCGTGGATTGCAAATCCTCT	50.0	50.0	57.6	58.2	169-170 bp	1.0	79.5
trnL1-trnC_p4	TAAGTCCGTTGCGTCTACCA	TAAGGCCGTGGATTGCAAAT	50.0	45.0	58.8	58.2	173-174 bp	1.0	76.9
trnL1-trnC_p5	TAAGTCCGTTGCGTCTACCA	CCGTGGATTGCAAATCCTCT	50.0	50.0	58.8	58.2	168-169 bp	1.0	76.3