Hotspot

trnG1-psbI

LSC · 35771-35921 · 151 bp

Back to Saccharina

Pi 0.0651

MarkerSeek score 65.60

Primer available yes

Label trnG1-psbI

Feature Metadata

label_name: trnG1-psbI
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 19
pi: 0.0651
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG1-psbI_p1	GTTGCGCTCTACCACTGAG	ACCCAAACCGAAAAGATTTCG	57.9	42.9	58.3	57.4	206-216 bp	1.0	64.1
trnG1-psbI_p2	GTTGCGCTCTACCACTGAG	CCCAAACCGAAAAGATTTCGA	57.9	42.9	58.3	57.1	205-215 bp	1.0	61.9
trnG1-psbI_p3	GTTGCGCTCTACCACTGAG	AACCCAAACCGAAAAGATTTCG	57.9	40.9	58.3	58.0	207-217 bp	1.0	60.9
trnG1-psbI_p4	CAAGGTTGCGCTCTACCAC	ACCCAAACCGAAAAGATTTCG	57.9	42.9	58.8	57.4	210-220 bp	1.0	59.3
trnG1-psbI_p5	GTTGCGCTCTACCACTGAG	CCCAAACCGAAAAGATTTCGAA	57.9	40.9	58.3	57.8	205-215 bp	1.0	59.3