Hotspot

trnT-UGU-trnL-UAA

LSC · 46304-47036 · 733 bp

Back to Sabulina

Pi 0.0775

MarkerSeek score 81.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 56
pi: 0.0775
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	789 bp	1.0	84.4
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.8	57.8	821 bp	1.0	83.3
trnT-UGU-trnL-UAA_p3	CTAACCTCTGAGCTAAGCGG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.5	57.8	781 bp	1.0	81.6
trnT-UGU-trnL-UAA_p4	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	791 bp	1.0	81.3
trnT-UGU-trnL-UAA_p5	TGCGATGCTCTAACCTCTGA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.5	57.8	790 bp	1.0	81.3