Hotspot

psaA-pafI

LSC · 40892-42080 · 1189 bp

Back to Sabulina

Pi 0.0665

MarkerSeek score 70.10

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 1189
variable_sites: 47
pi: 0.0665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.59
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TTTTGACTTCTGGTTCCGGC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.7	58.0	851-1321 bp	1.0	81.7
psaA-pafI_p2	TTTTGACTTCTGGTTCCGGC	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.7	57.8	784-1254 bp	1.0	80.8
psaA-pafI_p3	TTTTGACTTCTGGTTCCGGC	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.7	58.3	847-1317 bp	1.0	79.9
psaA-pafI_p4	TTTTGACTTCTGGTTCCGGC	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.7	58.3	849-1319 bp	1.0	79.9
psaA-pafI_p5	TTTGACTTCTGGTTCCGGC	ATCAAGCCGCTGAGTATTGG	52.6	50.0	58.0	58.0	850-1320 bp	1.0	79.0