Hotspot

trnG-GCC-trnfM-CAU

LSC · 35566-35756 · 191 bp

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Pi 0.118
MarkerSeek score 80.40
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
191
variable_sites
22
pi
0.118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG GTTCAAATCCTGTCTCCGCA 50.0 50.0 57.8 58.2 256-283 bp 1.0 85.1
trnG-GCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.8 57.8 290-317 bp 1.0 84.0
trnG-GCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 289-316 bp 1.0 84.0
trnG-GCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG ATTCGTCCCCAGTCCTTTTG 50.0 50.0 57.8 57.8 334-361 bp 1.0 83.3
trnG-GCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG CATTCGTCCCCAGTCCTTTT 50.0 50.0 57.8 57.8 335-362 bp 1.0 83.2