Hotspot

psbC-trnS-UGA

LSC · 34415-34633 · 219 bp

Back to Sabulina

Pi 0.155

MarkerSeek score 79.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 34
pi: 0.155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.4	58.0	384-422 bp	1.0	83.6
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	GTTGGAGAGATGGCTGAGTG	45.0	55.0	58.4	58.0	388-426 bp	1.0	83.5
psbC-trnS-UGA_p3	AGCTGCAGCAGGATTTGAAA	GCTGAGTGGTTGATAGCTCC	45.0	55.0	58.4	58.1	376-414 bp	1.0	83.5
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	369-407 bp	1.0	83.5
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GGCTGAGTGGTTGATAGCTC	45.0	55.0	58.4	58.1	377-415 bp	1.0	83.4