Hotspot

trnT-GGU-psbD

LSC · 30786-31983 · 1198 bp

Back to Sabulina

Pi 0.0707

MarkerSeek score 84.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 78
pi: 0.0707
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CTCGACGTAACCAGTCATCC	55.0	55.0	58.1	57.8	1261-1332 bp	1.0	86.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CTCGACGTAACCAGTCATCC	50.0	55.0	58.1	57.8	1264-1335 bp	1.0	86.6
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CTCGACGTAACCAGTCATCC	50.0	55.0	58.3	57.8	1238-1309 bp	1.0	85.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TCGACGTAACCAGTCATCCA	55.0	50.0	58.1	58.5	1260-1331 bp	1.0	84.6
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TCGACGTAACCAGTCATCCA	50.0	50.0	58.1	58.5	1263-1334 bp	1.0	84.4