Hotspot

trnE-UUC-trnT-GGU

LSC · 30131-30713 · 583 bp

Back to Sabulina

Pi 0.112

MarkerSeek score 87.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 63
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	670-678 bp	1.0	88.9
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	668-676 bp	1.0	88.9
trnE-UUC-trnT-GGU_p3	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	647-655 bp	1.0	88.8
trnE-UUC-trnT-GGU_p4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	649-657 bp	1.0	88.7
trnE-UUC-trnT-GGU_p5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	667-675 bp	1.0	88.4