Hotspot

trnD-GUC-trnY-GUA

LSC · 29472-29891 · 420 bp

Back to Sabulina

Pi 0.104

MarkerSeek score 86.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 42
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	476-491 bp	1.0	83.1
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	475-490 bp	1.0	83.1
trnD-GUC-trnY-GUA_p3	ATTGGTTGTTGGATCCGTCG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	537-552 bp	1.0	82.1
trnD-GUC-trnY-GUA_p4	ATTGGTTGTTGGATCCGTCG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	536-551 bp	1.0	82.1
trnD-GUC-trnY-GUA_p5	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	497-512 bp	1.0	81.8