Hotspot

psbM-trnD-GUC

LSC · 28711-29397 · 687 bp

Back to Sabulina

Pi 0.0797

MarkerSeek score 84.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 53
pi: 0.0797
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAATAGCGCAGTAGCA	TCAATTGGTTAGAGCACCGC	42.9	50.0	57.5	58.6	797-804 bp	1.0	71.4
psbM-trnD-GUC_p2	GAATGAATAGCGCAGTAGCA	TCAATTGGTTAGAGCACCGC	45.0	50.0	56.0	58.6	796-803 bp	1.0	67.3
psbM-trnD-GUC_p3	AGAATGAATAGCGCAGTAGCA	AATTGGTTAGAGCACCGCC	42.9	52.6	57.5	58.1	795-802 bp	1.0	66.8
psbM-trnD-GUC_p4	AGAATGAATAGCGCAGTAGCAA	TCAATTGGTTAGAGCACCGC	40.9	50.0	58.2	58.6	797-804 bp	1.0	65.5
psbM-trnD-GUC_p5	GAATGAATAGCGCAGTAGCAA	TCAATTGGTTAGAGCACCGC	42.9	50.0	56.8	58.6	796-803 bp	1.0	65.3