Hotspot

trnC-GCA-petN

LSC · 27354-27927 · 574 bp

Back to Sabulina

Pi 0.0676
MarkerSeek score 81.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
574
variable_sites
38
pi
0.0676
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 ATTCCCCAGTTCAAATCCGG GCCCAAGCGAGACTTACAAT 50.0 50.0 57.9 58.3 624-629 bp 1.0 83.1
trnC-GCA-petN_p2 ATTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 57.9 57.6 686-691 bp 1.0 81.9
trnC-GCA-petN_p3 ATTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAG 50.0 50.0 57.9 58.9 633-638 bp 1.0 77.9
trnC-GCA-petN_p4 CCAGTTCAAATCCGGGTGTC GCCCAAGCGAGACTTACAAT 55.0 50.0 58.8 58.3 619-624 bp 1.0 77.7
trnC-GCA-petN_p5 ATTCCCCAGTTCAAATCCGG GACTACCATTAAAGCAGCCCA 50.0 47.6 57.9 58.0 640-645 bp 1.0 77.1