Hotspot

atpH-atpI

LSC · 13300-14008 · 709 bp

Back to Sabulina

Pi 0.0809

MarkerSeek score 76.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 45
pi: 0.0809
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCGGCAGAAATCAGTGGATT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	58.9	57.9	668-787 bp	1.0	80.7
atpH-atpI_p2	GCGGCAGAAATCAGTGGATT	CGAATCCATGGAGGGTCATC	50.0	55.0	58.9	57.8	638-757 bp	1.0	80.5
atpH-atpI_p3	GCGGCAGAAATCAGTGGATT	TACCTGTCATGTTCCTCGGA	50.0	50.0	58.9	57.8	717-836 bp	1.0	79.6
atpH-atpI_p4	CGGCAGAAATCAGTGGATTC	TTTTGCAACTTTAGCCGCAG	50.0	45.0	56.9	57.9	667-786 bp	1.0	78.9
atpH-atpI_p5	CGGCAGAAATCAGTGGATTC	CGAATCCATGGAGGGTCATC	50.0	55.0	56.9	57.8	637-756 bp	1.0	78.8