Hotspot

rps16-trnQ-UUG

LSC · 6030-6662 · 633 bp

Back to Sabulina

Pi 0.0925

MarkerSeek score 80.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 53
pi: 0.0925
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GAGAACTAGCCCACCTTTCG	CTTACAGCAGCTTGCCAAAC	55.0	50.0	58.0	57.9	1377-1392 bp	1.0	87.5
rps16-trnQ-UUG_p2	GAGAACTAGCCCACCTTTCG	GGATAATGCGGGTTGTGTCT	55.0	50.0	58.0	58.0	1543 bp	0.5	59.6
rps16-trnQ-UUG_p3	TGTTCCGGAAGCCTTTATCG	GGATAATGCGGGTTGTGTCT	50.0	50.0	58.0	58.0	1853 bp	0.5	59.5
rps16-trnQ-UUG_p4	TTCCGGAAGCCTTTATCGTG	GGATAATGCGGGTTGTGTCT	50.0	50.0	58.0	58.0	1851 bp	0.5	59.5
rps16-trnQ-UUG_p5	GTTCCGGAAGCCTTTATCGT	GGATAATGCGGGTTGTGTCT	50.0	50.0	58.0	58.0	1852 bp	0.5	59.5